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PDLIM7 Polyclonal antibody
PDLIM7 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10221-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SKOV-3 cells, HeLa cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 4 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
10221-1-AP targets PDLIM7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PDLIM7 fusion protein Ag0264 相同性解析による交差性が予測される生物種 |
Full Name | PDZ and LIM domain 7 (enigma) |
Calculated molecular weight | 55/60 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC001093 |
Gene symbol | PDLIM7 |
Gene ID (NCBI) | 9260 |
RRID | AB_2161789 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.
Protocols
Product Specific Protocols | |
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WB protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
IHC protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
IF protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
IP protocol for PDLIM7 antibody 10221-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
EMBO Rep Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification. | ||
Cell Biosci TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1 | ||
J Bone Miner Res LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity. | ||
Front Immunol PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB. | ||
J Endod Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions. | ||
J Orthop Res LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tongbin (Verified Customer) (08-25-2020) | This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.
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FH Joleen (Verified Customer) (06-10-2019) | The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.
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