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Species Reactivity

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  • Featured Product
  • KD/KO Validated

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17080-1-AP

Synonyms

LMP1, LIM mineralization protein, ENIGMA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PDLIM7 Polyclonal antibody (17080-1-AP)

    WB analysis of HeLa using 17080-1-AP

    WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PDLIM7 Polyclonal antibody (17080-1-AP)

    WB analysis using 17080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PDLIM7 Polyclonal antibody (17080-1-AP)

    IP experiment of Jurkat using 17080-1-AP

    IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:1000) with Jurkat cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human colon using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human ovary using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human ovary using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human spleen using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human spleen using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human kidney using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human kidney using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human skin using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human skin using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue, human colon, human kidney tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17080-1-AP targets PDLIM7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM7 fusion protein Ag1334 相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 7 (enigma)
Calculated molecular weight 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC014521
Gene symbol PDLIM7
Gene ID (NCBI) 9260
RRIDAB_2161788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDLIM7 antibody 17080-1-APDownload protocol
IHC protocol for PDLIM7 antibody 17080-1-APDownload protocol
IP protocol for PDLIM7 antibody 17080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols