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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDP2 Polyclonal antibody

PDP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13404-1-AP

Synonyms

KIAA1348, PDP 2, PDP2, PDPC 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PDP2 Polyclonal antibody (13404-1-AP)

    WB analysis of mouse skeletal muscle using 13404-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PDP2 Polyclonal antibody (13404-1-AP)

    WB analysis of mouse kidney using 13404-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PDP2 Polyclonal antibody (13404-1-AP)

    WB analysis of HeLa using 13404-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13404-1-AP (PDP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PDP2 Polyclonal antibody (13404-1-AP)

    IHC staining of mouse brain using 13404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PDP2 Polyclonal antibody (13404-1-AP)

    IHC staining of mouse brain using 13404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PDP2 Polyclonal antibody (13404-1-AP)

    IHC staining of human lymphoma using 13404-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PDP2 Polyclonal antibody (13404-1-AP)

    IHC staining of human lymphoma using 13404-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13404-1-AP (PDP2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HeLa cells, mouse kidney tissue
Positive IHC detected inmouse brain tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13404-1-AP targets PDP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDP2 fusion protein Ag4257 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase phosphatase isoenzyme 2
Calculated molecular weight 529 aa, 60 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC028030
Gene symbol PDP2
Gene ID (NCBI) 57546
RRIDAB_10639505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDP2, is the Ca2+-insensitive isoform of pyruvate dehydrogenase phosphataseis and found in liver and adipose tissue. In adipose tissue,insulin reduces the concentration dependence of PDP2 activity for Mg2+(PMID:12676647).It catalyzes the dephosphorylation and concomitant reactivation of the mitochondrial pyruvate dehydrogenase multienzyme complex.It can be detected the mature (52 kDa) and the immature (60 kDa) bands in western blot.

Protocols

Product Specific Protocols
WB protocol for PDP2 antibody 13404-1-APDownload protocol
IHC protocol for PDP2 antibody 13404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Dev Cell

Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation.

Authors - Wanchun Yang
View Article
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
View Article