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  • Featured Product
  • KD/KO Validated

PDS5A Monoclonal antibody, PBS Only

PDS5A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8D4

Cat no : 67520-1-PBS

Synonyms

SCC 112, PIG54, PDS5, KIAA0648, Cell proliferation-inducing gene 54 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDS5A Monoclonal antibody (67520-1-Ig)

    WB analysis using 67520-1-Ig (same clone as 67520-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67520-1-Ig (PDS5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PDS5A Monoclonal antibody (67520-1-Ig)

    WB analysis of HeLa using 67520-1-Ig (same clone as 67520-1-PBS)

    WB result of PDS5A antibody (67520-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HeLa cells. This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDS5A Monoclonal antibody (67520-1-Ig)

    IHC staining of human breast cancer using 67520-1-Ig (same clone as 67520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDS5A Monoclonal antibody (67520-1-Ig)

    IHC staining of human breast cancer using 67520-1-Ig (same clone as 67520-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PDS5A Monoclonal antibody (67520-1-Ig)

    IF Staining of A431 using 67520-1-Ig (same clone as 67520-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDS5A Monoclonal antibody (67520-1-Ig)

    IF Staining of HepG2 using 67520-1-Ig (same clone as 67520-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67520-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67520-1-PBS targets PDS5A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDS5A fusion protein Ag11525 相同性解析による交差性が予測される生物種
Full Name PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
Calculated molecular weight 1297 aa, 147 kDa
Observed molecular weight130 kDa
GenBank accession numberBC009650
Gene symbol PDS5A
Gene ID (NCBI) 23244
RRIDAB_2882740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.