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KD/KO Validated

PDSS2 Polyclonal antibody

Name: PDSS2 Polyclonal antibody
Brand: Proteintech
SKU: 13544-1-AP

PDSS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13544-1-AP

Synonyms

C6orf210, DLP1, hDLP1, PDSS2

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of A375 using 13544-1-AP
WB result of PDSS2 antibody (13544-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDSS2 transfected A375 cells.
WB analysis of SGC-7901 using 13544-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13544-1-AP (PDSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 13544-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 13544-1-AP (PDSS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 13544-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 13544-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 13544-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13544-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13544-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13544-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 13544-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SGC-7901 cells, A375 cells
Positive IHC detected in	human kidney tissue, human liver cancer tissue, mouse liver tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IF	See 2 publications below

Product Information

13544-1-AP targets PDSS2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PDSS2 fusion protein Ag4487 相同性解析による交差性が予測される生物種
Full Name	prenyl (decaprenyl) diphosphate synthase, subunit 2
Calculated molecular weight	399 aa, 44 kDa
Observed molecular weight	27-30 kDa, 44-47 kDa
GenBank accession number	BC039906
Gene symbol	PDSS2
Gene ID (NCBI)	57107
RRID	AB_10598311
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDSS2, also named as C6orf210, is required for the synthesis of coenzyme Q10 (CoQ10), which is synthesized in the mitochondrial inner membrane and plays a vital role in the mitochondrial respiratory chain, pyrimidine nucleoside biosynthesis, and modulation of apoptosis (PMID:25330808). An increasing body of evidence suggests that PDSS2 is a tumor suppressor gene in certain cancers, such as melanoma, gastric cancer and non-small cell lung cancer. Besides, PDSS2 could be used as a prognostic biomarker for HCC (PMID:25780306). PDSS2 has 2 isoforms with MW of 44 kDa and 27 kDa while 13544-1-AP antibody detects the bands around 27-30 kDa and 44-47 kDa in SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for PDSS2 antibody 13544-1-AP	Download protocol
IHC protocol for PDSS2 antibody 13544-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. Authors - Agustín Hidalgo-Gutiérrez View Article
mouse	WB, IF	Aging Cell Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging. Authors - Assaf Ben-Meir View Article
human	WB	Neurol Genet Novel Homozygous Variant in COQ7 in Siblings With Hereditary Motor Neuropathy Authors - Ian C Smith View Article
human	WB	J Assist Reprod Genet PMID: 26552664 Authors - Yaakov Bentov View Article
human	WB	Mol Genet Metab Rep A novel COQ7 mutation causing primarily neuromuscular pathology and its treatment options Authors - Ying Wang View Article
mouse	IF	Antioxidants (Basel) Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model. Authors - Assaf Ben-Meir View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDSS2 Polyclonal antibody

PDSS2 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of A375 using 13544-1-AP

WB analysis of SGC-7901 using 13544-1-AP

WB analysis of A375 using 13544-1-AP

IHC staining of mouse liver using 13544-1-AP

IHC staining of human kidney using 13544-1-AP

IHC staining of rat kidney using 13544-1-AP

IHC staining of human liver cancer using 13544-1-AP

IHC staining of human liver cancer using 13544-1-AP

IHC staining of human liver cancer using 13544-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging.

Novel Homozygous Variant in COQ7 in Siblings With Hereditary Motor Neuropathy

PMID: 26552664

A novel COQ7 mutation causing primarily neuromuscular pathology and its treatment options

Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model.