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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDXDC1 Monoclonal antibody, PBS Only

PDXDC1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E6E2

Cat no : 68278-1-PBS

Synonyms

KIAA0251, LP8165, PDXDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDXDC1 Monoclonal antibody (68278-1-Ig)

    WB analysis using 68278-1-Ig (same clone as 68278-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68278-1-Ig (PDXDC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDXDC1 Monoclonal antibody (68278-1-Ig)

    WB analysis using 68278-1-Ig (same clone as 68278-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68278-1-Ig (PDXDC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PDXDC1 Monoclonal antibody (68278-1-Ig)

    IF Staining of MCF-7 using 68278-1-Ig (same clone as 68278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PDXDC1 antibody (68278-1-Ig, Clone: 1E6E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PDXDC1 Monoclonal antibody (68278-1-Ig)

    IF Staining of MCF-7 using 68278-1-Ig (same clone as 68278-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDXDC1 antibody (68278-1-Ig, Clone: 1E6E2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68278-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68278-1-PBS targets PDXDC1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDXDC1 fusion protein Ag15489 相同性解析による交差性が予測される生物種
Full Name pyridoxal-dependent decarboxylase domain containing 1
Calculated molecular weight 788 aa, 87 kDa
Observed molecular weight87 kDa
GenBank accession numberBC033748
Gene symbol PDXDC1
Gene ID (NCBI) 23042
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971).