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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDZD7 Polyclonal antibody

PDZD7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21084-1-AP

Synonyms

PDZK7, PDZ domain-containing protein 7, PDZ domain containing 7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDZD7 Polyclonal antibody (21084-1-AP)

    WB analysis using 21084-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21084-1-AP (PDZD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PDZD7 Polyclonal antibody (21084-1-AP)

    IHC staining of mouse eye using 21084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PDZD7 Polyclonal antibody (21084-1-AP)

    IHC staining of mouse eye using 21084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse ear tissue, mouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21084-1-AP targets PDZD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDZD7 fusion protein Ag15318 相同性解析による交差性が予測される生物種
Full Name PDZ domain containing 7
Calculated molecular weight 517 aa, 56 kDa
Observed molecular weight55 kDa
GenBank accession numberBC029054
Gene symbol PDZD7
Gene ID (NCBI) 79955
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDZD7, also known as PDZK7, is essential in organizing the USH2 complex at stereocilia ankle links and blocks inhibition of adenylate cyclase activity mediated by ADGRV1. PDZD7 encodes a PDZ domain-containing scaffold protein that is highly expressed in hair cells of the inner ear. PDZD7 is also expressed in the cilia of photoreceptors (PMID: 33530996). Dysfunction or mutations of PDZD7 result in Usher syndrome type 2 (USH2) and Autosomal recessive non-syndromic hearing loss(ARNSHL) (PMID: 29048736, 20440071).

Protocols

Product Specific Protocols
WB protocol for PDZD7 antibody 21084-1-APDownload protocol
IHC protocol for PDZD7 antibody 21084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols