Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
10507-2-AP targets PDZK1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDZK1 fusion protein Ag0787 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ domain containing 1 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 63-70 kDa |
| GenBank accession number | BC006496 |
| Gene Symbol | PDZK1 |
| Gene ID (NCBI) | 5174 |
| RRID | AB_2299495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T2W1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| IP protocol for PDZK1 antibody 10507-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Downregulation of vimentin expression increased drug resistance in ovarian cancer cells. | ||
Mol Med PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth. | ||
Mol Med Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37. | ||
Cell Div miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism
| ||
Aging (Albany NY) Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1
| ||
Bone Res PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function |