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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEA15 Recombinant antibody

PEA15 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241044H6

Cat no : 83958-3-RR

Synonyms

MAT1H, MAT1, HUMMAT1H, HMAT1, Astrocytic phosphoprotein PEA-15

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PEA15 Recombinant antibody (83958-3-RR)

    IF Staining of A431 using 83958-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PEA15 antibody (83958-3-RR, Clone: 241044H6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83958-3-RR

    Affinity and Kinetic Characterization of 83958-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83958-3-RR against Human PEA15 were performed. The affinity constant is 6.95 nM.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83958-3-RR is PEA15 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEA15 fusion protein Ag13782 相同性解析による交差性が予測される生物種
Full Name phosphoprotein enriched in astrocytes 15
Calculated molecular weight 130 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC002426
Gene symbol PEA15
Gene ID (NCBI) 8682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphoprotein Enriched in Astrocytes 15 kDa (PEA15), also known as PED-15, is a small, death effector-domain (DED)-containing protein that was recently demonstrated to inhibit tumor necrosis factor-α-induced apoptosis and to reverse the inhibition of integrin activation due to H-Ras. PEA15 is a 15 kDa protein that is highly expressed in the nervous system with particularly high levels in astrocytes and neurons of the hippocampus. PEA15 is a cytoplasmic protein that sits at an important junction in intracellular signalling and can regulate diverse cellular processes, such as proliferation and apoptosis, dependent upon stimulation.

Protocols

Product Specific Protocols
IF protocol for PEA15 antibody 83958-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols