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RKIP Polyclonal antibody
RKIP Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10575-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, mouse liver tissue, mouse testis tissue |
Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 3 publications below |
Product Information
10575-1-AP targets RKIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RKIP fusion protein Ag0864 相同性解析による交差性が予測される生物種 |
Full Name | phosphatidylethanolamine binding protein 1 |
Calculated molecular weight | 21 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC008714 |
Gene symbol | PEBP1 |
Gene ID (NCBI) | 5037 |
RRID | AB_2299521 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.
Protocols
Product Specific Protocols | |
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WB protocol for RKIP antibody 10575-1-AP | Download protocol |
IHC protocol for RKIP antibody 10575-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
FASEB J Menstrual fluid factors facilitate tissue repair: identification and functional action in endometrial and skin repair. | ||
Anticancer Res Differential Protein Expression of Lymph Node Metastases of Papillary Thyroid Carcinoma Harboring the BRAF Mutation. | ||
J Ethnopharmacol AR/RKIP pathway mediates the inhibitory effects of icariin on renal fibrosis and endothelial-to-mesenchymal transition in type 2 diabetic nephropathy
| ||
Medicine (Baltimore) Prognostic significance of RKIP, TGM2, and CMTM4 expression in oral squamous cell carcinoma |