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Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEBP1 Monoclonal antibody, PBS Only

PEBP1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4D11

Cat no : 66438-1-PBS

Synonyms

RKIP, PEBP 1, PEBP, PBP, Neuropolypeptide h3

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using PEBP1 Monoclonal antibody (66438-1-Ig)

    WB analysis of fetal human brain using 66438-1-Ig (same clone as 66438-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using PEBP1 Monoclonal antibody (66438-1-Ig)

    WB analysis of Neuro-2a using 66438-1-Ig (same clone as 66438-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using PEBP1 Monoclonal antibody (66438-1-Ig)

    WB analysis of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PEBP1 Monoclonal antibody (66438-1-Ig)

    WB analysis of rat brain using 66438-1-Ig (same clone as 66438-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66438-1-Ig (PEBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PEBP1 Monoclonal antibody (66438-1-Ig)

    IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 10x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PEBP1 Monoclonal antibody (66438-1-Ig)

    IHC staining of human prostate cancer using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66438-1-Ig (PEBP1 Antibody) at dilution of 1:500 (under 40x lens). proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using PEBP1 Monoclonal antibody (66438-1-Ig)

    IF Staining of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PEBP1 Monoclonal antibody (66438-1-Ig)

    IF Staining of A549 using 66438-1-Ig (same clone as 66438-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using PEBP1 antibody (66438-1-Ig, Clone: 2D4D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-12 cells using PEBP1 Monoclonal antibody (66438-1-Ig)

    FC experiment of PC-12 using 66438-1-Ig (same clone as 66438-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human PEBP1 (66438-1-Ig, Clone:2D4D11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66438-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66438-1-PBS targets PEBP1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEBP1 fusion protein Ag0864 相同性解析による交差性が予測される生物種
Full Name phosphatidylethanolamine binding protein 1
Calculated molecular weight 21 kDa
Observed molecular weight 23-27 kDa
GenBank accession numberBC008714
Gene symbol PEBP1
Gene ID (NCBI) 5037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Raf kinase inhibitor protein (RKIP), also termed phosphatidylethanolamine binding protein PEBP1, was initially identified as a potent inhibitor of Raf-1/MEK/ERK, NF-kB, and G-protein-coupled receptor signaling pathways. Later RKIP has been further identified as a metastasis suppressor and its loss of expression has been reported in various cancers. Its expression has been proposed as a prognostic marker for patients diagnosed with the above cancers.