CD31 Monoclonal antibody
CD31 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2A1E2
Cat no : 66065-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, THP-1 cells, human placenta tissue |
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 7 publications below |
IHC | See 6 publications below |
IF | See 11 publications below |
Product Information
The immunogen of 66065-1-Ig is CD31 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD31 fusion protein Ag1787 相同性解析による交差性が予測される生物種 |
Full Name | platelet/endothelial cell adhesion molecule |
Calculated molecular weight | 83 kDa |
Observed molecular weight | 120-130 kDa |
GenBank accession number | BC022512 |
Gene symbol | CD31 |
Gene ID (NCBI) | 5175 |
RRID | AB_11042590 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD31 antibody 66065-1-Ig | Download protocol |
IHC protocol for CD31 antibody 66065-1-Ig | Download protocol |
IF protocol for CD31 antibody 66065-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomaterials An iRGD-conjugated prodrug micelle with blood-brain-barrier penetrability for anti-glioma therapy. | ||
Small Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle. | ||
Theranostics Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer. | ||
Theranostics Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis. | ||
Cancer Lett CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma. |