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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PECI Polyclonal antibody

PECI Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20383-1-AP

Synonyms

ACBD2, D3,D2 enoyl CoA isomerase, DBI related protein 1, Dodecenoyl CoA isomerase, DRS 1, DRS1, ECI2, HCA88, KIAA0536, PECI

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PECI Polyclonal antibody (20383-1-AP)

    WB analysis of human placenta using 20383-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20383-1-AP (PECI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using PECI Polyclonal antibody (20383-1-AP)

    IHC staining of human heart using 20383-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20383-1-AP (PECI antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using PECI Polyclonal antibody (20383-1-AP)

    IHC staining of human hepatocirrhosis using 20383-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20383-1-AP (PECI antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman heart tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20383-1-AP is PECI Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PECI fusion protein Ag14211 相同性解析による交差性が予測される生物種
Full Name peroxisomal D3,D2-enoyl-CoA isomerase
Calculated molecular weight 394 aa, 44 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC034702
Gene symbol PECI
Gene ID (NCBI) 10455
RRIDAB_10696313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PECI antibody 20383-1-APDownload protocol
IHC protocol for PECI antibody 20383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Omega

Proteomics Analysis Provides Insights into the Role of Lipid Metabolism in T2DM-Related Sarcopenia

Authors - Jingying Wu
View Article