PEG10 Polyclonal antibody
PEG10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 14412-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, L02 cells, PC-13 cells, mouse liver tissue |
Positive IHC detected in | mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 3 publications below |
Product Information
14412-1-AP targets PEG10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PEG10 fusion protein Ag5754 相同性解析による交差性が予測される生物種 |
Full Name | paternally expressed 10 |
Calculated molecular weight | 80 kDa |
Observed molecular weight | 50 kDa, 100 kDa |
GenBank accession number | BC050659 |
Gene symbol | PEG10 |
Gene ID (NCBI) | 23089 |
RRID | AB_10694427 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for PEG10 antibody 14412-1-AP | Download protocol |
IHC protocol for PEG10 antibody 14412-1-AP | Download protocol |
IF protocol for PEG10 antibody 14412-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Pharmacol Sin Transcriptional regulator CTR9 promotes hepatocellular carcinoma progression and metastasis via increasing PEG10 transcriptional activity. | ||
bioRxiv Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties | ||
Elife UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS | ||
Ann Med A 12-gene panel in estimating hormone-treatment responses of castration-resistant prostate cancer patients generated using a combined analysis of bulk and single-cell sequencing data | ||
J Exp Clin Cancer Res Targeting PEG10 as a novel therapeutic approach to overcome CDK4/6 inhibitor resistance in breast cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Harihar (Verified Customer) (01-28-2024) | The antibody is excellent for Western blotting. There is some non-specific nuclear staining in case of IF at the dilutions we have tested at.
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FH Jordan (Verified Customer) (09-03-2019) | The antibody works well in our condition. MCF-7 cell lines. 1/1000 in TBS-T 5%BSA.
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