PEG10 Polyclonal antibody

PEG10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14412-1-AP

Synonyms

EDR, KIAA1051e, KIAA1051, HB1, HB 1


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Tested Applications

Positive WB detected inHepG2 cells, L02 cells, PC-13 cells, mouse liver tissue
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14412-1-AP targets PEG10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEG10 fusion protein Ag5754 相同性解析による交差性が予測される生物種
Full Name paternally expressed 10
Calculated molecular weight 80 kDa
Observed molecular weight 50 kDa, 100 kDa
GenBank accession numberBC050659
Gene symbol PEG10
Gene ID (NCBI) 23089
RRIDAB_10694427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PEG10 antibody 14412-1-APDownload protocol
IHC protocol for PEG10 antibody 14412-1-APDownload protocol
IF protocol for PEG10 antibody 14412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Cell

Cellular architecture of human brain metastases.

Authors - Hugo Gonzalez
humanWB

Acta Pharmacol Sin

Transcriptional regulator CTR9 promotes hepatocellular carcinoma progression and metastasis via increasing PEG10 transcriptional activity.

Authors - Bin Zhang
humanWB,IF

bioRxiv

Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties

Authors - Harihar M Mohan
humanIF

Elife

UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS

Authors - Holly H Black
humanWB

Ann Med

A 12-gene panel in estimating hormone-treatment responses of castration-resistant prostate cancer patients generated using a combined analysis of bulk and single-cell sequencing data

Authors - Juanlan Huang
humanIHC

J Exp Clin Cancer Res

Targeting PEG10 as a novel therapeutic approach to overcome CDK4/6 inhibitor resistance in breast cancer

Authors - Nar Bahadur Katuwal

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Harihar (Verified Customer) (01-28-2024)

The antibody is excellent for Western blotting. There is some non-specific nuclear staining in case of IF at the dilutions we have tested at.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:300 (IF), 1:3000 (WB)
  • Cell Tissue Type: HeLa cells, Kolf2 iPSCs
PEG10 Antibody Western Blot,Immunofluorescence validation (1:300 (IF), 1:3000 (WB) dilution) in HeLa cells, Kolf2 iPSCs (Cat no:14412-1-AP)
FH

Jordan (Verified Customer) (09-03-2019)

The antibody works well in our condition. MCF-7 cell lines. 1/1000 in TBS-T 5%BSA.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000