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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEG3 Polyclonal antibody (23569-1-AP)

    WB analysis using 23569-1-AP (same clone as 23569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23569-1-AP (PEG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PEG3 Polyclonal antibody (23569-1-AP)

    IHC staining of human placenta using 23569-1-AP (same clone as 23569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEG3 Polyclonal antibody (23569-1-AP)

    IHC staining of mouse brain using 23569-1-AP (same clone as 23569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PEG3 Polyclonal antibody (23569-1-AP)

    IHC staining of human placenta using 23569-1-AP (same clone as 23569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23569-1-AP (PEG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23569-1-PBS in a different storage buffer formulation.

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PEG3 Polyclonal antibody, PBS Only

PEG3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 23569-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23569-1-PBS targets PEG3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEG3 fusion protein Ag20132 相同性解析による交差性が予測される生物種
Full Name paternally expressed 3
Calculated molecular weight 1588 aa, 181 kDa
Observed molecular weight200-220 kDa
GenBank accession numberBC150272
Gene Symbol PEG3
Gene ID (NCBI) 5178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9GZU2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Paternally expressed 3 (PEG3) was responsible for Kras/Erk- and mTOR-driven HCC (hepatocellular carcinoma) (PMID: 36451866). PEG3 can regulate the physiological processes related to energy homeostasis found in multiple tissues, such as the brain, testis, ovary, and placenta (PMID: 32729618). Epigenetic silencing and loss of imprinting of PEG3 are implicated in glioma tumorigenesis (PMID: 32231463).