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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PELP1 Monoclonal antibody (67050-1-Ig)

    WB analysis using 67050-1-Ig (same clone as 67050-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using PELP1 Monoclonal antibody (67050-1-Ig)

    WB analysis of T-47D using 67050-1-Ig (same clone as 67050-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PELP1 Monoclonal antibody (67050-1-Ig)

    WB analysis of Jurkat using 67050-1-Ig (same clone as 67050-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67050-1-Ig (PELP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PELP1 Monoclonal antibody (67050-1-Ig)

    IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PELP1 Monoclonal antibody (67050-1-Ig)

    IHC staining of human cervical cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PELP1 Monoclonal antibody (67050-1-Ig)

    IHC staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PELP1 Monoclonal antibody (67050-1-Ig)

    IHC staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67050-1-Ig (PELP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using PELP1 Monoclonal antibody (67050-1-Ig)

    IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using PELP1 Monoclonal antibody (67050-1-Ig)

    IF Staining of human breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse breast cancer using PELP1 Monoclonal antibody (67050-1-Ig)

    IF Staining of mouse breast cancer using 67050-1-Ig (same clone as 67050-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using PELP1 antibody (67050-1-Ig, Clone: 1B11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PELP1 Monoclonal antibody (67050-1-Ig)

    FC experiment of MCF-7 using 67050-1-Ig (same clone as 67050-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PELP1 (67050-1-Ig, Clone:1B11E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67050-1-PBS in a different storage buffer formulation.

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PELP1 Monoclonal antibody, PBS Only

PELP1 Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA
Cat No. 67050-1-PBS
Clone No.1B11E4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Synonyms

1B11E4, HMX3, MNAR, Modulator of non-genomic activity of estrogen receptor, Proline-, glutamic acid- and leucine-rich protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67050-1-PBS targets PELP1 in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PELP1 fusion protein Ag25729 相同性解析による交差性が予測される生物種
Full Name proline, glutamate and leucine rich protein 1
Calculated molecular weight 1130 aa, 120 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC069058
Gene Symbol PELP1
Gene ID (NCBI) 27043
RRIDAB_2882363
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IZL8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PELP1 was first identified as a 160 kDa protein in a screen for Src homology 2 (SH2) domain-binding proteins. PELP1 is overexpressed in 60-80% of breast tumors and plays important roles in both ER genomic and non-genomic signaling. In vivo, PELP1 subcellular localization is primarily nuclear in normal breast tissue, but it is localized to the cytoplasm in about 40% of invasive breast tumors. In the nucleus, PELP1 interacts with a number of transcription factors. The proto-oncogenic functions of PELP1 involve different cellular processes including epigenetic modifications leading to ER transactivation and breast cancer progression. Furthermore, PELP1 activates kinase cascades in the cytoplasm such as MAPK activation via c-Src and PI3K signaling.