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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEMT Recombinant antibody

PEMT Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242150D5

Cat no : 85099-1-RR

Synonyms

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using PEMT Recombinant antibody (85099-1-RR)

    WB analysis of SH-SY5Y using 85099-1-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    WB analysis of mouse liver using 85099-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    IHC staining of mouse liver using 85099-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    IHC staining of mouse liver using 85099-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85099-1-RR targets PEMT in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEMT fusion protein Ag8034 相同性解析による交差性が予測される生物種
Full Name phosphatidylethanolamine N-methyltransferase
Calculated molecular weight 26 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC050593
Gene symbol PEMT
Gene ID (NCBI) 10400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).

Protocols

Product Specific Protocols
WB protocol for PEMT antibody 85099-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols