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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis of HEK-293 using 12218-1-AP

    WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis using 12218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis of mouse skin using 12218-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis of HEK-293 using 12218-1-AP

    WB result of PEPD antibody (12218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEPD transfected HEK-293 cells.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis of MDA-MB-453s using 12218-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis using 12218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis using 12218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis of HeLa using 12218-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis of HepG2 using 12218-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PEPD Polyclonal antibody (12218-1-AP)

    WB analysis using 12218-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12218-1-AP (PEPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PEPD Polyclonal antibody (12218-1-AP)

    IHC staining of human lung cancer using 12218-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12218-1-AP (PEPD antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PEPD Polyclonal antibody (12218-1-AP)

    IF Staining of HepG2 using 12218-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12218-1-AP (PEPD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

PEPD Polyclonal antibody

PEPD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12218-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

X-Pro dipeptidase, Xaa-Pro dipeptidase, PROLIDASE, PRD, EC:3.4.13.9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MDA-MB-453s cells, mouse skin tissue, HeLa cells, HepG2 cells, SH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:100-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12218-1-AP targets PEPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEPD fusion protein Ag2842 相同性解析による交差性が予測される生物種
Full Name peptidase D
Calculated molecular weight 493 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC015027
Gene Symbol PEPD
Gene ID (NCBI) 5184
RRIDAB_2161517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Protocols

Product Specific Protocols
WB protocol for PEPD antibody 12218-1-APDownload protocol
IHC protocol for PEPD antibody 12218-1-APDownload protocol
IF protocol for PEPD antibody 12218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

BMC Med Genet

Analysis of positional candidate genes in the AAA1 susceptibility locus for abdominal aortic aneurysms on chromosome 19.

Authors - Lillvis John H JH
View Article
humanIHC

Arch Med Res

Relationship Between Echocardiographically Evaluated Aortic Stiffness and Prolidase Activity in Aortic Tissue of Patients with Critical Coronary Artery Disease.

Authors - Aydemir Koçarslan
View Article
humanWB

Nat Commun

Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA

Authors - Ya Nan Zhu
View Article