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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEPD Monoclonal antibody, PBS Only

PEPD Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H2A1

Cat no : 67202-1-PBS

Synonyms

Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis using 67202-1-Ig (same clone as 67202-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis of HEK-293 using 67202-1-Ig (same clone as 67202-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis of human placenta using 67202-1-Ig (same clone as 67202-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using PEPD Monoclonal antibody (67202-1-Ig)

    WB analysis of NCCIT using 67202-1-Ig (same clone as 67202-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PEPD Monoclonal antibody (67202-1-Ig)

    IHC staining of human breast cancer using 67202-1-Ig (same clone as 67202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PEPD Monoclonal antibody (67202-1-Ig)

    IHC staining of human breast cancer using 67202-1-Ig (same clone as 67202-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67202-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67202-1-PBS targets PEPD in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEPD fusion protein Ag28713 相同性解析による交差性が予測される生物種
Full Name peptidase D
Calculated molecular weight 493 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC015027
Gene symbol PEPD
Gene ID (NCBI) 5184
RRIDAB_2882495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)