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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PER2 (isoform 1) Monoclonal antibody (67513-1-Ig)

    WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PER2 (isoform 1) Monoclonal antibody (67513-1-Ig)

    WB analysis using 67513-1-Ig (same clone as 67513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67513-1-Ig (PER2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PER2 (isoform 1) Monoclonal antibody (67513-1-Ig)

    IF Staining of HeLa using 67513-1-Ig (same clone as 67513-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PER2 (isoform 1) antibody (67513-1-Ig, Clone: 2E6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67513-1-PBS in a different storage buffer formulation.

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PER2 (isoform 1) Monoclonal antibody, PBS Only

PER2 (isoform 1) Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67513-1-PBS
Clone No.2E6C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PER2, PER 2, hPER2, FASPS, 2E6C3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67513-1-PBS targets PER2 (isoform 1) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PER2 (isoform 1) fusion protein Ag29489 相同性解析による交差性が予測される生物種
Full Name period homolog 2 (Drosophila)
Calculated molecular weight 137 kDa
Observed molecular weight150~160 kDa
GenBank accession numberNM_022817
Gene Symbol PER2
Gene ID (NCBI) 8864
RRIDAB_2882734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15055
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).