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  • Featured Product
  • KD/KO Validated

PERK/EIF2AK3 Polyclonal antibody, PBS Only

PERK/EIF2AK3 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 24390-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    IHC staining of human liver cancer using 24390-1-AP (same clone as 24390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    IHC staining of human liver cancer using 24390-1-AP (same clone as 24390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    FC experiment of HeLa using 24390-1-AP (same clone as 24390-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (24390-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24390-1-PBS targets PERK/EIF2AK3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PERK/EIF2AK3 fusion protein Ag18254 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 1116 aa, 125 kDa
Observed molecular weight140 kDa
GenBank accession numberBC126354
Gene symbol PERK
Gene ID (NCBI) 9451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).