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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    WB analysis using 24390-1-AP (same clone as 24390-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    IHC staining of human liver cancer using 24390-1-AP (same clone as 24390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 10x lens).. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    IHC staining of human liver cancer using 24390-1-AP (same clone as 24390-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24390-1-AP (PERK/EIF2AK3 antibody) at dilution of 1:200 (under 40x lens).. This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PERK/EIF2AK3 Polyclonal antibody (24390-1-AP)

    FC experiment of HeLa using 24390-1-AP (same clone as 24390-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PERK/EIF2AK3 (24390-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24390-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PERK/EIF2AK3 Polyclonal antibody, PBS Only

PERK/EIF2AK3 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 24390-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24390-1-PBS targets PERK/EIF2AK3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PERK/EIF2AK3 fusion protein Ag18254 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 1116 aa, 125 kDa
Observed molecular weight140 kDa
GenBank accession numberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
RRIDAB_2879521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).