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PERK/EIF2AK3 Monoclonal antibody

PERK/EIF2AK3 Monoclonal Antibody for WB, ELISA
Cat No. 68482-1-Ig
Clone No.3G3F2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, ELISA

DKFZp781H1925, EIF2AK3, HRI, HsPEK, Pancreatic eIF2 alpha kinase, PEK, PERK, WRS

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inDaudi cells, HSC-T6 cells, Ramos cells, Jurkat cells, K-562 cells, HuH-7 cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

68482-1-Ig targets PERK/EIF2AK3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PERK/EIF2AK3 fusion protein Ag18254 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Calculated molecular weight 1116 aa, 125 kDa
Observed molecular weight140 kDa
GenBank accession numberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
RRIDAB_3085193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PERK, also named as EIF2AK3 or PEK, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and GCN2 subfamily. It acts as a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. PERK and EIF2AK4 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases (PMID: 21638082). Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS). The molecular mass of PERK is 125 kDa and can be detected as 140 kDa or 170kDa due to some modifications such as glycosylation and phosphorylation (PMID: 20851775, 24371088, 21703327).

Protocols

Product Specific Protocols
WB protocol for PERK/EIF2AK3 antibody 68482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Int J Mol Sci

Evaluation of Cross-Talk and Alleviate Potential of Cytotoxic Factors Induced by Deoxynivalenol in IPEC-J2 Cells Interference with Curcumin

Authors - Qiyuan Wang
humanWB

Cancer Gene Ther

N6-methyladenosine modification of RIMS binding protein 2 promotes head and neck squamous cell carcinoma proliferation and radiotherapy tolerance through endoplasmic reticulum stress

Authors - Xinyu Sun
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