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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEX12 Polyclonal antibody

PEX12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27011-1-AP

Synonyms

PEX, Peroxisome assembly protein 12, Peroxisome assembly factor 3, Peroxin-12, Peroxin 12

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PEX12 Polyclonal antibody (27011-1-AP)

    WB analysis of MCF-7 using 27011-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PEX12 Polyclonal antibody (27011-1-AP)

    WB analysis of PC-3 using 27011-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PEX12 Polyclonal antibody (27011-1-AP)

    WB analysis of HeLa using 27011-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PEX12 Polyclonal antibody (27011-1-AP)

    WB analysis of A549 using 27011-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PEX12 Polyclonal antibody (27011-1-AP)

    WB analysis of HepG2 using 27011-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27011-1-AP (PEX12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, HeLa cells, HepG2 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27011-1-AP targets PEX12 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX12 fusion protein Ag25500 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 12
Calculated molecular weight 41 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC031085
Gene symbol PEX12
Gene ID (NCBI) 5193
RRIDAB_2880720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxisome assembly protein 12 (PEX12), also known as Peroxisome assembly factor 3 (PAF-3), is an integral peroxisomal membrane protein that is essential for peroxisomal matrix protein import and is one of only two peroxins known to interact with the ligand-binding domain of PEX5. Mutations in human PEX12 result in Zellweger syndrome, a lethal neurological disorder, and implicate the zinc ring domain in PEX12 function (PMID: 12456682). Pex12 exhibits protein-Ub ligase activity with specificity for the E2 protein Pex4 and Ubc4 (PMID: 19687296).

Protocols

Product Specific Protocols
WB protocol for PEX12 antibody 27011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Commun Biol

PEX3 promotes regenerative repair after myocardial injury in mice through facilitating plasma membrane localization of ITGB3

Authors - Jia-Teng Sun
View Article