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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PEX13 Polyclonal antibody (26649-1-AP)

    WB analysis of Jurkat using 26649-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26649-1-AP (PEX13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PEX13 Polyclonal antibody (26649-1-AP)

    WB analysis of HeLa using 26649-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26649-1-AP (PEX13 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX13 Polyclonal antibody (26649-1-AP)

    IHC staining of human kidney using 26649-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX13 Polyclonal antibody (26649-1-AP)

    IHC staining of human kidney using 26649-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26649-1-AP (PEX13 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX13 Polyclonal antibody (26649-1-AP)

    IF Staining of HeLa using 26649-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26649-1-AP (PEX13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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PEX13 Polyclonal antibody

PEX13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26649-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NALD, Peroxin 13, PEX13, ZWS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

26649-1-AP targets PEX13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX13 fusion protein Ag24400 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 13
Calculated molecular weight 403 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC067090
Gene Symbol PEX13
Gene ID (NCBI) 5194
RRIDAB_2880588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92968
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PEX13 antibody 26649-1-APDownload protocol
IHC protocol for PEX13 antibody 26649-1-APDownload protocol
IF protocol for PEX13 antibody 26649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Authors - Michael S Dahabieh
View Article
humanWB

Biol Chem

Comparison of human PEX knockout cell lines suggests a dual role of PEX1 in peroxisome biogenesis

Authors - Julia Ott
View Article
humanWB

Nat Commun

Modulation of peroxisomal import by the PEX13 SH3 domain and a proximal FxxxF binding motif

Authors - Stefan Gaussmann
View Article