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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    WB analysis using 80196-1-RR (same clone as 80196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    IHC staining of human liver cancer using 80196-1-RR (same clone as 80196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    IHC staining of human liver cancer using 80196-1-RR (same clone as 80196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    IHC staining of human kidney using 80196-1-RR (same clone as 80196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    IHC staining of human kidney using 80196-1-RR (same clone as 80196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80196-1-RR (PEX14 (human specific) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    IF Staining of HeLa using 80196-1-RR (same clone as 80196-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 (human specific) antibody (80196-1-RR, Clone: 6K5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PEX14 (human specific) Recombinant antibody (80196-1-RR)

    FC experiment of HeLa using 80196-1-RR (same clone as 80196-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (human specific) (80196-1-RR, Clone:6K5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80196-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80196-1-RR

    Affinity and Kinetic Characterization of 80196-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80196-1-RR against PEX14 (human specific) were performed. The affinity constant is 0.613 nM.

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PEX14 (human specific) Recombinant antibody, PBS Only

PEX14 (human specific) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 80196-1-PBS
Clone No.6K5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PEX14, PEX, Peroxin-14, Peroxin 14, NAPP2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80196-1-PBS targets PEX14 (human specific) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PEX14 (human specific) fusion protein Ag0932 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight57 kDa
GenBank accession numberBC006327
Gene Symbol PEX14
Gene ID (NCBI) 5195
RRIDAB_2918874
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75381
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

This antibody is specific to human PEX14. It can't detect mouse or rat PEX14.