Validation Data Gallery
Tested Applications
| Positive WB detected in | human liver tissue, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14816-1-AP targets PEX16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, arabidopsis |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PEX16 fusion protein Ag6566 相同性解析による交差性が予測される生物種 |
| Full Name | peroxisomal biogenesis factor 16 |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC000467 |
| Gene Symbol | PEX16 |
| Gene ID (NCBI) | 9409 |
| RRID | AB_2162250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Y5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX16 antibody 14816-1-AP | Download protocol |
| IF protocol for PEX16 antibody 14816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.
| ||
Nat Commun Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. | ||
Cell Death Differ Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis. | ||
Proc Natl Acad Sci U S A Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells. | ||
Cell Death Differ Peroxisomes protect lymphoma cells from HDAC inhibitor-mediated apoptosis. |