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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEX16 Monoclonal antibody (68261-1-Ig)

    WB analysis using 68261-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68261-1-Ig (PEX16 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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PEX16 Monoclonal antibody

PEX16 Monoclonal Antibody for WB, ELISA
Cat No. 68261-1-Ig
Clone No.2D7G4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Synonyms

Peroxin 16, PEX16

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68261-1-Ig targets PEX16 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEX16 fusion protein Ag6574 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 16
Calculated molecular weight 39 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000467
Gene Symbol PEX16
Gene ID (NCBI) 9409
RRIDAB_2935346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y5Y5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).

Protocols

Product Specific Protocols
WB protocol for PEX16 antibody 68261-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols