PEX19 Polyclonal antibody
PEX19 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 14713-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, mouse heart tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IF | See 2 publications below |
Product Information
14713-1-AP targets PEX19 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, arabidopsis |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PEX19 fusion protein Ag6434 相同性解析による交差性が予測される生物種 |
| Full Name | peroxisomal biogenesis factor 19 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC000496 |
| Gene symbol | PEX19 |
| Gene ID (NCBI) | 5824 |
| RRID | AB_2162265 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PEX19 antibody 14713-1-AP | Download protocol |
| IHC protocol for PEX19 antibody 14713-1-AP | Download protocol |
| IP protocol for PEX19 antibody 14713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling. | ||
Cell Death Differ Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis. | ||
Cell Commun Signal TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions | ||
Cell Mol Life Sci Dietary cysteine and methionine promote peroxisome elevation and fat loss by induction of CG33474 expression in Drosophila adipose tissue |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Susanne (Verified Customer) (07-03-2023) | very good in western blot, membrane blockes with milkpowder in TBST no non-specitif bands detection of ~60ng purified recomb. human Pex19 mit HRP-conjugated secondary antibody and ECL
 |
FH Tom (Verified Customer) (09-08-2021) | Very good antibody - no non-specific bands. Membrane blocked with BSA in TBS.
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