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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PEX19 Monoclonal antibody (68555-1-Ig)

    WB analysis using 68555-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68555-1-Ig (PEX19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX19 Monoclonal antibody (68555-1-Ig)

    IF Staining of HeLa using 68555-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using PEX19 Monoclonal antibody (68555-1-Ig)

    IF Staining of Jurkat using 68555-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PEX19 antibody (68555-1-Ig, Clone: 3H2C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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PEX19 Monoclonal antibody

PEX19 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68555-1-Ig
Clone No.3H2C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

PMPI, PMP1, PEX, Peroxin-19, OK/SW-cl.22

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, Pig liver tissue, Rat liver tissue, Mouse liver tissue
Positive IF/ICC detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68555-1-Ig targets PEX19 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PEX19 fusion protein Ag6858 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 19
Calculated molecular weight 33 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC000496
Gene Symbol PEX19
Gene ID (NCBI) 5824
RRIDAB_3085258
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. PEX19 gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs) (PMID: 14709540). PEX19 may bind newly synthesized PMPs and facilitate their insertion into the peroxisome membrane (PMID: 107044440. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14) (PMID:20683989) (PMID:10051604).

Protocols

Product Specific Protocols
WB protocol for PEX19 antibody 68555-1-IgDownload protocol
IF protocol for PEX19 antibody 68555-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols