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  • Featured Product
  • KD/KO Validated

PEX5 Polyclonal antibody

PEX5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12545-1-AP

Synonyms

PTS1 BP, PEX, Peroxisome receptor 1, Peroxisomal C-terminal targeting signal import receptor, Peroxin-5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using PEX5 Polyclonal antibody (12545-1-AP)

    WB analysis of HEK-293 using 12545-1-AP

    WB result of PEX5 antibody (12545-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX5 transfected HEK-293 cells.

  • Western Blot (WB) analysis of human brain tissue using PEX5 Polyclonal antibody (12545-1-AP)

    WB analysis of human brain using 12545-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PEX5 Polyclonal antibody (12545-1-AP)

    WB analysis of L02 using 12545-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using PEX5 Polyclonal antibody (12545-1-AP)

    WB analysis of human kidney using 12545-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PEX5 Polyclonal antibody (12545-1-AP)

    WB analysis of mouse kidney using 12545-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PEX5 Polyclonal antibody (12545-1-AP)

    WB analysis of mouse brain using 12545-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12545-1-AP (PEX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using PEX5 Polyclonal antibody (12545-1-AP)

    IP experiment of mouse kidney using 12545-1-AP

    IP result of anti-PEX5 (IP:12545-1-AP, 4ug; Detection:12545-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Polyclonal antibody (12545-1-AP)

    IHC staining of human kidney using 12545-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12545-1-AP (PEX5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PEX5 Polyclonal antibody (12545-1-AP)

    IHC staining of human kidney using 12545-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12545-1-AP (PEX5 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEX5 Polyclonal antibody (12545-1-AP)

    IHC staining of mouse brain using 12545-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12545-1-AP (PEX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PEX5 Polyclonal antibody (12545-1-AP)

    IHC staining of mouse brain using 12545-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12545-1-AP (PEX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PEX5 Polyclonal antibody (12545-1-AP)

    IF Staining of HeLa using 12545-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12545-1-AP (PEX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using PEX5 Polyclonal antibody (12545-1-AP)

    FC experiment of HEK-293 using 12545-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug PEX5 Polyclonal antibody (12545-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inhuman brain tissue, L02 cells, mouse kidney tissue, mouse brain tissue, human kidney tissue, HEK-293 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12545-1-AP targets PEX5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX5 fusion protein Ag3268 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 5
Calculated molecular weight 631 aa, 70 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC010621
Gene symbol PEX5
Gene ID (NCBI) 5830
RRIDAB_2268102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).

Protocols

Product Specific Protocols
WB protocol for PEX5 antibody 12545-1-APDownload protocol
IHC protocol for PEX5 antibody 12545-1-APDownload protocol
IF protocol for PEX5 antibody 12545-1-APDownload protocol
IP protocol for PEX5 antibody 12545-1-APDownload protocol
FC protocol for PEX5 antibody 12545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 and autophagy in response to ROS.

Authors - Zhang Jiangwei J
  • KD Validated
View Article
humanWB, IF

Nat Cell Biol

ATM functions at the peroxisome to induce pexophagy in response to ROS.

Authors - Jiangwei Zhang
View Article
humanWB,IF,IP

Autophagy

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Authors - Michael S Dahabieh
View Article
humanWB,IF

Cell Mol Life Sci

Dietary cysteine and methionine promote peroxisome elevation and fat loss by induction of CG33474 expression in Drosophila adipose tissue

Authors - Meng Liu
View Article
humanWB

Hum Mol Genet

A novel type of rhizomelic chondrodysplasia punctata, RCDP5, is caused by loss of the PEX5 long isoform.

Authors - Tuva Barøy
View Article

Biochim Biophys Acta Mol Cell Biol Lipids

Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.

Authors - Zhihui Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Zhihui (Verified Customer) (12-12-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T And mouse astrocytes
PEX5 Antibody Western Blot, validation (1:1000 dilution) in HEK293T And mouse astrocytes (Cat no:12545-1-AP)