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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PEX7 Polyclonal antibody

PEX7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20614-1-AP

Synonyms

Peroxin 7, PEX7, PTS2 receptor, PTS2R, RCDP1, RD

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using PEX7 Polyclonal antibody (20614-1-AP)

    WB analysis of MDA-MB-453s using 20614-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20614-1-AP (PEX7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PEX7 Polyclonal antibody (20614-1-AP)

    WB analysis of MDA-MB-453s using 20614-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20614-1-AP (PEX7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inMDA-MB-453s cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 20614-1-AP is PEX7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX7 fusion protein Ag14666 相同性解析による交差性が予測される生物種
Full Name peroxisomal biogenesis factor 7
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC006268
Gene symbol PEX7
Gene ID (NCBI) 5191
RRIDAB_10694826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX7, also known as peroxisomal targeting signal 2 (PTS2) receptor, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively. PEX7 plays an essential role in peroxisomal protein import by binding to the N-terminal PTS2-type peroxisomal targeting signal. PEX7 defects are associated with peroxisome biogenesis disorder complementation group 11 (PBD-CG11), rhizomelic chondrodysplasia punctata type 1 (RCDP1) and Refsum disease.

Protocols

Product Specific Protocols
WB protocol for PEX7 antibody 20614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Genet

Tysnd1 deficiency in mice interferes with the peroxisomal localization of PTS2 enzymes, causing lipid metabolic abnormalities and male infertility.

Authors - Mizuno Yumi Y
View Article
mouseWB

PLoS One

Pharmacological inhibition of catalase induces peroxisome leakage and suppression of LPS induced inflammatory response in Raw 264.7 cell.

Authors - Yizhu Mu
View Article