Validation Data Gallery
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Tested Applications
| Positive WB detected in | Human peripheral blood platelets tissue, mouse spleen tissue |
| Positive IP detected in | mouse serum |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
21157-1-AP targets PF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PF4 fusion protein Ag12345 相同性解析による交差性が予測される生物種 |
| Full Name | platelet factor 4 |
| Calculated molecular weight | 101 aa, 11 kDa |
| Observed molecular weight | 10 kDa |
| GenBank accession number | BC093965 |
| Gene Symbol | PF4 |
| Gene ID (NCBI) | 5196 |
| RRID | AB_2878819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02776 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PF4 antibody 21157-1-AP | Download protocol |
| IHC protocol for PF4 antibody 21157-1-AP | Download protocol |
| IF protocol for PF4 antibody 21157-1-AP | Download protocol |
| IP protocol for PF4 antibody 21157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol CXCL4:NLRP3-mediated pyroptosis product that regulates cardiac fibrosis | ||
Toxicol Appl Pharmacol Synergistic anticancer effects of ginsenoside CK and gefitinib against gefitinib-resistant NSCLC by regulating the balance of angiogenic factors through HIF-1α/VEGF | ||
J Virol Platelet factor 4-derived C15 peptide broadly inhibits enteroviruses by disrupting viral attachment | ||
J Biol Chem ELF4 facilitates innate host defenses against Plasmodium by activating transcription of Pf4 and Ppbp | ||
Cell Biosci Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis. |