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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PFAS Recombinant antibody (83074-3-RR)

    IF Staining of HepG2 using 83074-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PFAS antibody (83074-3-RR, Clone: 230422D12 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83074-3-RR

    Affinity and Kinetic Characterization of 83074-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83074-3-RR against Human PFAS were performed. The affinity constant is 0.58 nM.

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PFAS Recombinant antibody

PFAS Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83074-3-RR
Clone No.230422D12

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Synonyms

FGAMS, FGARAT, KIAA0361, PFAS, PFAS protein, PURL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83074-3-RR targets PFAS in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PFAS fusion protein Ag20162 相同性解析による交差性が予測される生物種
Full Name phosphoribosylformylglycinamidine synthase
Calculated molecular weight 1338 aa, 145 kDa
GenBank accession numberBC167158
Gene Symbol PFAS
Gene ID (NCBI) 5198
RRIDAB_3670794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15067
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for PFAS antibody 83074-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols