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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFDN1 Polyclonal antibody

PFDN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11033-2-AP

Synonyms

PDF, PFD1, PFDN1, prefoldin subunit 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using PFDN1 Polyclonal antibody (11033-2-AP)

    WB analysis of human brain using 11033-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11033-2-AP (PFDN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using PFDN1 Polyclonal antibody (11033-2-AP)

    WB analysis of human colon using 11033-2-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 11033-2-AP (PFDN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PFDN1 Polyclonal antibody (11033-2-AP)

    IHC staining of human colon cancer using 11033-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11033-2-AP (PFDN1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, human colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

11033-2-AP targets PFDN1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFDN1 fusion protein Ag1506 相同性解析による交差性が予測される生物種
Full Name prefoldin subunit 1
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC011869
Gene symbol PFDN1
Gene ID (NCBI) 5201
RRIDAB_2283690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFDN1 antibody 11033-2-APDownload protocol
IHC protocol for PFDN1 antibody 11033-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Jundishapur J Microbiol

Analysis of Subcellular Prefoldin 1 Redistribution During Rabies Virus Infection.

Authors - Jinyang Zhang
View Article