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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFDN2 Polyclonal antibody

PFDN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13053-1-AP

Synonyms

PFD2, HSPC231

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using PFDN2 Polyclonal antibody (13053-1-AP)

    WB analysis of L02 using 13053-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 13053-1-AP (PFDN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using PFDN2 Polyclonal antibody (13053-1-AP)

    WB analysis of Neuro-2a using 13053-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 13053-1-AP (PFDN2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PFDN2 Polyclonal antibody (13053-1-AP)

    IHC staining of human lung cancer using 13053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13053-1-AP (PFDN2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PFDN2 Polyclonal antibody (13053-1-AP)

    IHC staining of human liver cancer using 13053-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13053-1-AP (PFDN2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PFDN2 Polyclonal antibody (13053-1-AP)

    IHC staining of human liver cancer using 13053-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13053-1-AP (PFDN2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using PFDN2 Polyclonal antibody (13053-1-AP)

    IF Staining of L02 using 13053-1-AP

    Immunofluorescent analysis of L02 cells using 13053-1-AP (PFDN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inL02 cells, Neuro-2a cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13053-1-AP targets PFDN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFDN2 fusion protein Ag3720 相同性解析による交差性が予測される生物種
Full Name prefoldin subunit 2
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight 17-20 kDa
GenBank accession numberBC012464
Gene symbol PFDN2
Gene ID (NCBI) 5202
RRIDAB_2877905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFDN2 antibody 13053-1-APDownload protocol
IHC protocol for PFDN2 antibody 13053-1-APDownload protocol
IF protocol for PFDN2 antibody 13053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols