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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PFDN5 Polyclonal antibody (15078-1-AP)

    WB analysis of HepG2 using 15078-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PFDN5 Polyclonal antibody (15078-1-AP)

    WB analysis of mouse pancreas using 15078-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PFDN5 Polyclonal antibody (15078-1-AP)

    WB analysis of PC-3 using 15078-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15078-1-AP (PFDN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PFDN5 Polyclonal antibody (15078-1-AP)

    IF Staining of HepG2 using 15078-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PFDN5 antibody (15078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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PFDN5 Polyclonal antibody

PFDN5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15078-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

PFD5, MM1, c-Myc-binding protein Mm-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, mouse pancreas tissue, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15078-1-AP targets PFDN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFDN5 fusion protein Ag7150 相同性解析による交差性が予測される生物種
Full Name prefoldin subunit 5
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC003373
Gene Symbol PFDN5
Gene ID (NCBI) 5204
RRIDAB_2162703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99471
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFDN5 antibody 15078-1-APDownload protocol
IF protocol for PFDN5 antibody 15078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Brain Sci

Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest

Authors - Qiang Zhang
View Article