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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKL Monoclonal antibody (68385-1-Ig)

    WB analysis using 68385-1-Ig (same clone as 68385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68385-1-Ig (PFKL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of human lung cancer using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of mouse liver using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of human ovary tumor using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of human ovary tumor using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of human liver using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PFKL Monoclonal antibody (68385-1-Ig)

    IHC staining of human liver using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68385-1-Ig (PFKL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using PFKL Monoclonal antibody (68385-1-Ig)

    IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using PFKL Monoclonal antibody (68385-1-Ig)

    IF Staining of HuH-7 using 68385-1-Ig (same clone as 68385-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PFKL antibody (68385-1-Ig, Clone: 1H2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using PFKL Monoclonal antibody (68385-1-Ig)

    FC experiment of HEK-293 using 68385-1-Ig (same clone as 68385-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug PFKL Monoclonal antibody (68385-1-Ig, Clone:1H2G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68385-1-PBS in a different storage buffer formulation.

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PFKL Monoclonal antibody, PBS Only

PFKL Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68385-1-PBS
Clone No.1H2G2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

PFK B, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, liver type, 6-phosphofructokinase type B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68385-1-PBS targets PFKL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PFKL fusion protein Ag8250 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, liver
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC008964
Gene Symbol PFKL
Gene ID (NCBI) 5211
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17858
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PFKL (6-phosphofructokinase, liver type) is also named as PFK-B and belongs to the phosphofructokinase family. The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. In human beings, PFK exists as a system of three subunits: these are the muscle(PFKM), liver(PFKL), and platelet(PFKP) PFKs (PMID: 2139864). It has 2 isoforms produced by alternative splicing.