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  • Featured Product
  • KD/KO Validated

PFKM Polyclonal antibody

PFKM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30326-1-AP

Synonyms

PFK-A, PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKM Polyclonal antibody (30326-1-AP)

    WB analysis using 30326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PFKM Polyclonal antibody (30326-1-AP)

    WB analysis of HEK-293 using 30326-1-AP

    WB result of PFKM antibody (30326-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using PFKM Polyclonal antibody (30326-1-AP)

    WB analysis using 30326-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30326-1-AP (PFKM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PFKM Polyclonal antibody (30326-1-AP)

    IHC staining of mouse skeletal muscle using 30326-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PFKM Polyclonal antibody (30326-1-AP)

    IHC staining of mouse skeletal muscle using 30326-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30326-1-AP (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PFKM Polyclonal antibody (30326-1-AP)

    IF Staining of HepG2 using 30326-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed 48-well HepG2 cells using PFKM antibody (30326-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, Raji cells, MCF-7 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in48-well HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30326-1-AP targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKM fusion protein Ag30840 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, muscle
Observed molecular weight 75-85 kDa
GenBank accession numberBC021203
Gene symbol PFKM
Gene ID (NCBI) 5213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa.

Protocols

Product Specific Protocols
WB protocol for PFKM antibody 30326-1-APDownload protocol
IHC protocol for PFKM antibody 30326-1-APDownload protocol
IF protocol for PFKM antibody 30326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols