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KD/KO Validated

PFKM Polyclonal antibody

Name: PFKM Polyclonal antibody
Brand: Proteintech
SKU: 55028-1-AP

PFKM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55028-1-AP

Synonyms

PFK-A, PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 55028-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55028-1-AP
WB result of PFKM antibody (55028-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HeLa cells.
WB analysis of HEK-293 using 55028-1-AP
WB result of PFKM antibody (55028-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKM transfected HEK-293 cells.
WB analysis of human skeletal muscle using 55028-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55028-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 55028-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55028-1-AP (PFKM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 55028-1-AP
IP result of anti-PFKM (IP:55028-1-AP, 4ug; Detection:55028-1-AP 1:8000) with mouse heart tissue lysate 1680 ug.
IP experiment of mouse liver using 55028-1-AP
IP result of anti-PFKM (IP:55028-1-AP, 4ug; Detection:55028-1-AP 1:300) with mouse liver tissue lysate 5200ug.
IHC staining of human skeletal muscle using 55028-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55028-1-AP (PFKM Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 55028-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55028-1-AP (PFKM Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse skeletal muscle using 55028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 55028-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55028-1-AP (PFKM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 55028-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (55028-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Cat No. 55028-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human skeletal muscle tissue, HepG2 cells, human brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue
Positive IP detected in	mouse heart tissue, mouse liver tissue
Positive IHC detected in	mouse skeletal muscle tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 60 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

55028-1-AP targets PFKM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	phosphofructokinase, muscle
Calculated molecular weight	85 kDa
Observed molecular weight	75-85 kDa
GenBank accession number	NM_000289
Gene symbol	PFKM
Gene ID (NCBI)	5213
RRID	AB_10858390
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa. The antibody is specific to PFKM.

Protocols

Product Specific Protocols
WB protocol for PFKM antibody 55028-1-AP	Download protocol
IHC protocol for PFKM antibody 55028-1-AP	Download protocol
IF protocol for PFKM antibody 55028-1-AP	Download protocol
IP protocol for PFKM antibody 55028-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P Authors - Yuan-Li Ai View Article
human	WB	Nature Mechanical regulation of glycolysis via cytoskeleton architecture. Authors - Jin Suk Park View Article
human	WB	Nat Methods A large-scale targeted proteomics assay resource based on an in vitro human proteome. Authors - Masaki Matsumoto View Article
mouse	IF	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	WB	Biomaterials Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance. Authors - Meiling Wu View Article
human	WB	Cancer Res Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer. Authors - Jiao Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFKM Polyclonal antibody

PFKM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 55028-1-AP

WB analysis of HeLa using 55028-1-AP

WB analysis of HEK-293 using 55028-1-AP

WB analysis of human skeletal muscle using 55028-1-AP

WB analysis of HepG2 using 55028-1-AP

WB analysis of human brain using 55028-1-AP

IP experiment of mouse heart using 55028-1-AP

IP experiment of mouse liver using 55028-1-AP

IHC staining of human skeletal muscle using 55028-1-AP

IHC staining of human skeletal muscle using 55028-1-AP

IHC staining of mouse skeletal muscle using 55028-1-AP

IHC staining of mouse skeletal muscle using 55028-1-AP

IF Staining of HeLa using 55028-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Mechanical regulation of glycolysis via cytoskeleton architecture.

A large-scale targeted proteomics assay resource based on an in vitro human proteome.

A glycolytic shift in Schwann cells supports injured axons.

Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.

Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer.