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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKM Recombinant antibody (84281-5-RR)

    WB analysis using 84281-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using PFKM Recombinant antibody (84281-5-RR)

    WB analysis of mouse skeletal muscle using 84281-5-RR

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 84281-5-RR (PFKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PFKM Recombinant antibody (84281-5-RR)

    IHC staining of mouse skeletal muscle using 84281-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PFKM Recombinant antibody (84281-5-RR)

    IHC staining of mouse skeletal muscle using 84281-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84281-5-RR (PFKM antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PFKM Recombinant antibody (84281-5-RR)

    IF Staining of HeLa using 84281-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PFKM antibody (84281-5-RR, Clone: 241598B2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PFKM Recombinant antibody (84281-5-RR)

    IF Staining of HepG2 using 84281-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PFKM antibody (84281-5-RR, Clone: 241598B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84281-5-RR

    Affinity and Kinetic Characterization of 84281-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84281-5-RR against Human PFKM were performed. The affinity constant is 3.01 nM.

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PFKM Recombinant antibody

PFKM Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84281-5-RR
Clone No.241598B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PFK A, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type, 6-phosphofructokinase type A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, PC-3 cells, Raji cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84281-5-RR targets PFKM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PFKM fusion protein Ag30840 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, muscle
Observed molecular weight 75-85 kDa
GenBank accession numberBC021203
Gene Symbol PFKM
Gene ID (NCBI) 5213
RRIDAB_3671827
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM cause glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular weights of 85, 82, and 93 kDa.

Protocols

Product Specific Protocols
WB protocol for PFKM antibody 84281-5-RRDownload protocol
IHC protocol for PFKM antibody 84281-5-RRDownload protocol
IF protocol for PFKM antibody 84281-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols