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KD/KO Validated

PFKP Polyclonal antibody

Name: PFKP Polyclonal antibody
Brand: Proteintech
SKU: 13389-1-AP

PFKP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13389-1-AP

Synonyms

PFKF, PFK-C, EC:2.7.1.11, ATP-PFK, ATP-dependent 6-phosphofructokinase, platelet type

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 13389-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 13389-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 13389-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13389-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13389-1-AP (PFKP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 13389-1-AP
IP result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:4000) with Jurkat cells lysate 2280 ug.
IP experiment of mouse heart using 13389-1-AP
IP result of anti-PFKP (IP:13389-1-AP, 4ug; Detection:13389-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
IHC staining of mouse heart using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human spleen using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13389-1-AP (PFKP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 13389-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13389-1-AP (PFKP Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of BxPC-3 using 13389-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using PFKP antibody (13389-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13389-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (13389-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13389-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, BxPC-3 cells, human heart tissue, NIH/3T3 cells, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue, Jurkat cells, MCF-7 cells, Raji cells
Positive IP detected in	Jurkat cells, mouse heart tissue
Positive IHC detected in	mouse heart tissue, human lung cancer tissue, human heart tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 27 publications below
IHC	See 4 publications below
IF	See 2 publications below
IP	See 2 publications below

Product Information

13389-1-AP targets PFKP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PFKP fusion protein Ag4047 相同性解析による交差性が予測される生物種
Full Name	phosphofructokinase, platelet
Calculated molecular weight	784 aa, 86 kDa
Observed molecular weight	80-86 kDa
GenBank accession number	BC029138
Gene symbol	PFKP
Gene ID (NCBI)	5214
RRID	AB_2252278
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).

Protocols

Product Specific Protocols
WB protocol for PFKP antibody 13389-1-AP	Download protocol
IHC protocol for PFKP antibody 13389-1-AP	Download protocol
IF protocol for PFKP antibody 13389-1-AP	Download protocol
IP protocol for PFKP antibody 13389-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P Authors - Yuan-Li Ai View Article
mouse	WB	Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation Authors - Xiaolei Lin View Article
mouse	WB,IF	Nat Metab Time of exercise differentially impacts bone growth in mice Authors - Shaoling Yu View Article
human	WB	Mol Cell R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Authors - Ying Qing KD Validated View Article
human	WB	Stem Cell Reports The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway. Authors - Zihao Sun View Article
mouse	WB	Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. Authors - Lei Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFKP Polyclonal antibody

PFKP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 13389-1-AP

WB analysis using 13389-1-AP

WB analysis using 13389-1-AP

WB analysis of human heart using 13389-1-AP

IP experiment of Jurkat using 13389-1-AP

IP experiment of mouse heart using 13389-1-AP

IHC staining of mouse heart using 13389-1-AP

IHC staining of mouse heart using 13389-1-AP

IHC staining of human spleen using 13389-1-AP

IHC staining of human spleen using 13389-1-AP

IHC staining of human lung cancer using 13389-1-AP

IHC staining of human lung cancer using 13389-1-AP

IHC staining of human heart using 13389-1-AP

IHC staining of human heart using 13389-1-AP

IF Staining of BxPC-3 using 13389-1-AP

IF Staining of HeLa using 13389-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Augmentation of scleral glycolysis promotes myopia through histone lactylation

Time of exercise differentially impacts bone growth in mice

R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis.

The Long Noncoding RNA Lncenc1 Maintains Naive States of Mouse ESCs by Promoting the Glycolysis Pathway.

Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis.