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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKP Monoclonal antibody (68129-1-Ig)

    WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PFKP Monoclonal antibody (68129-1-Ig)

    WB analysis using 68129-1-Ig (same clone as 68129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68129-1-Ig (PFKP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PFKP Monoclonal antibody (68129-1-Ig)

    IF Staining of HeLa using 68129-1-Ig (same clone as 68129-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using PFKP Monoclonal antibody (68129-1-Ig)

    IF Staining of BxPC-3 using 68129-1-Ig (same clone as 68129-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using PFKP antibody (68129-1-Ig, Clone: 1H4D4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68129-1-PBS in a different storage buffer formulation.

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PFKP Monoclonal antibody, PBS Only

PFKP Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68129-1-PBS
Clone No.1H4D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

1H4D4, 6-phosphofructokinase type C, ATP-dependent 6-phosphofructokinase, platelet type, ATP-PFK, EC:2.7.1.11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68129-1-PBS targets PFKP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PFKP fusion protein Ag25206 相同性解析による交差性が予測される生物種
Full Name phosphofructokinase, platelet
Calculated molecular weight 784 aa, 86 kDa
Observed molecular weight80-86 kDa
GenBank accession numberBC029138
Gene Symbol PFKP
Gene ID (NCBI) 5214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01813
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PFKP(6-phosphofructokinase, platelet type) is also named as PFKF, PFK-C and belongs to the phosphofructokinase family. This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK (Francke (1983)). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The observed molecular weight of PFKP monomer is 80-86 kDa. The molecular mass of PFKP dimer is 171 kDa (PMID: 28607489).