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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    WB analysis of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    WB analysis of rat brain using 60094-2-Ig (same clone as 60094-2-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    IHC staining of human lung cancer using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    IHC staining of human lung cancer using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    IHC staining of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    IHC staining of mouse brain using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    IHC staining of human skeletal muscle using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using PFN2 Monoclonal antibody (60094-2-Ig)

    IHC staining of human skeletal muscle using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PFN2 Monoclonal antibody (60094-2-Ig)

    IF Staining of HeLa using 60094-2-Ig (same clone as 60094-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PFN2 antibody (60094-2-Ig, Clone: 2H7C12 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60094-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PFN2 Monoclonal antibody, PBS Only

PFN2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60094-2-PBS
Clone No.2H7C12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Prifilin 2, Profilin II, profilin 2, PFL, D3S1319E

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60094-2-PBS targets PFN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PFN2 fusion protein Ag5043 相同性解析による交差性が予測される生物種
Full Name profilin 2
Calculated molecular weight 140 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC018049
Gene Symbol PFN2
Gene ID (NCBI) 5217
RRIDAB_2163215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35080
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PFN2 (profilin 2) is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. The MW of this protein is 15 kDa, and this antibody specially recognises the 15 kDa protein.