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  • Featured Product
  • KD/KO Validated

PGAM1 Monoclonal antibody, PBS Only

PGAM1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H2A9

Cat no : 67470-1-PBS

Synonyms

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGAM1 Monoclonal antibody (67470-1-Ig)

    WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PGAM1 Monoclonal antibody (67470-1-Ig)

    WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGAM1 Monoclonal antibody (67470-1-Ig)

    IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGAM1 Monoclonal antibody (67470-1-Ig)

    IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PGAM1 Monoclonal antibody (67470-1-Ig)

    IF Staining of HepG2 using 67470-1-Ig (same clone as 67470-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGAM1 antibody (67470-1-Ig, Clone: 2H2A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67470-1-PBS targets PGAM1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGAM1 fusion protein Ag9250 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate mutase 1 (brain)
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC011678
Gene symbol PGAM1
Gene ID (NCBI) 5223
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.