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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGAM5 Monoclonal antibody, PBS Only

PGAM5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8B9

Cat no : 68116-1-PBS

Synonyms

BXLBv68, PGAM5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PGAM5 Monoclonal antibody (68116-1-Ig)

    WB analysis using 68116-1-Ig (same clone as 68116-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit brain tissue using PGAM5 Monoclonal antibody (68116-1-Ig)

    WB analysis of rabbit brain using 68116-1-Ig (same clone as 68116-1-PBS)

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using PGAM5 Monoclonal antibody (68116-1-Ig)

    WB analysis of pig brain using 68116-1-Ig (same clone as 68116-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68116-1-Ig (PGAM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGAM5 Monoclonal antibody (68116-1-Ig)

    IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PGAM5 Monoclonal antibody (68116-1-Ig)

    IHC staining of human liver cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PGAM5 Monoclonal antibody (68116-1-Ig)

    IHC staining of human lung cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PGAM5 Monoclonal antibody (68116-1-Ig)

    IHC staining of human lung cancer using 68116-1-Ig (same clone as 68116-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68116-1-Ig (PGAM5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68116-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68116-1-PBS targets PGAM5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PGAM5 fusion protein Ag28195 相同性解析による交差性が予測される生物種
Full Name phosphoglycerate mutase family member 5
Calculated molecular weight 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC008196
Gene symbol PGAM5
Gene ID (NCBI) 192111
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.