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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGBD1 Polyclonal antibody

PGBD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25539-1-AP

Synonyms

SCAND4, PiggyBac transposable element-derived protein 1, PGBD 1, hucep-4, HUCEP4

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PGBD1 Polyclonal antibody (25539-1-AP)

    WB analysis of HeLa using 25539-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PGBD1 Polyclonal antibody (25539-1-AP)

    WB analysis of K-562 using 25539-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PGBD1 Polyclonal antibody (25539-1-AP)

    WB analysis of mouse liver using 25539-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PGBD1 Polyclonal antibody (25539-1-AP)

    WB analysis of HepG2 using 25539-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25539-1-AP (PGBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PGBD1 Polyclonal antibody (25539-1-AP)

    IHC staining of human kidney using 25539-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGBD1 Polyclonal antibody (25539-1-AP)

    IHC staining of human testis using 25539-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PGBD1 Polyclonal antibody (25539-1-AP)

    IHC staining of mouse testis using 25539-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25539-1-AP (PGBD1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PGBD1 Polyclonal antibody (25539-1-AP)

    IHC staining of human kidney using 25539-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGBD1 Polyclonal antibody (25539-1-AP)

    IHC staining of human testis using 25539-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25539-1-AP (PGBD1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PGBD1 Polyclonal antibody (25539-1-AP)

    IF Staining of HepG2 using 25539-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGBD1 antibody (25539-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, mouse liver tissue
Positive IHC detected inmouse testis tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25539-1-AP targets PGBD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGBD1 fusion protein Ag19837 相同性解析による交差性が予測される生物種
Full Name piggyBac transposable element derived 1
Calculated molecular weight 809 aa, 93 kDa
Observed molecular weight 85-90 kDa
GenBank accession numberBC128585
Gene symbol PGBD1
Gene ID (NCBI) 84547
RRIDAB_2880123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PGBD1 antibody 25539-1-APDownload protocol
IHC protocol for PGBD1 antibody 25539-1-APDownload protocol
IF protocol for PGBD1 antibody 25539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols