Pepsinogen II Polyclonal antibody
Pepsinogen II Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16881-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human stomach tissue, mouse stomach tissue |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
16881-1-AP targets Pepsinogen II in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Pepsinogen II fusion protein Ag10332 相同性解析による交差性が予測される生物種 |
Full Name | progastricsin (pepsinogen C) |
Calculated molecular weight | 388 aa, 42 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC073740 |
Gene symbol | PGC |
Gene ID (NCBI) | 5225 |
RRID | AB_10596919 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for Pepsinogen II antibody 16881-1-AP | Download protocol |
IHC protocol for Pepsinogen II antibody 16881-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |