PGC Polyclonal antibody
PGC Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, MOUSE, rat and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 28532-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse stomach tissue, rat stomach tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
28532-1-AP targets PGC in WB, IHC, ELISA applications and shows reactivity with Human, MOUSE, rat samples.
| Tested Reactivity | Human, MOUSE, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PGC fusion protein Ag29347 相同性解析による交差性が予測される生物種 |
| Full Name | progastricsin (pepsinogen C) |
| Calculated molecular weight | 388 aa, 42 kDa |
| GenBank accession number | BC073740 |
| Gene symbol | PGC |
| Gene ID (NCBI) | 5225 |
| RRID | AB_2918173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PGC antibody 28532-1-AP | Download protocol |
| IHC protocol for PGC antibody 28532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Biosci Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy | ||
Transgenic Res The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells |
