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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PGC1a Monoclonal antibody (66369-1-Ig)

    WB analysis using 66369-1-Ig (same clone as 66369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PGC1a Monoclonal antibody (66369-1-Ig)

    WB analysis of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

    WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using PGC1a Monoclonal antibody (66369-1-Ig)

    WB analysis of HSC-T6 using 66369-1-Ig (same clone as 66369-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PGC1a Monoclonal antibody (66369-1-Ig)

    IF Staining of HeLa using 66369-1-Ig (same clone as 66369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGC1a antibody (66369-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66369-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

PGC1a Monoclonal antibody, PBS Only

PGC1a Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66369-1-PBS
Clone No.1C1B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PPARGC1A, PGC 1v, PGC 1(alpha), PGC 1 alpha, Ligand effect modulator 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66369-1-PBS targets PGC1a in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Calculated molecular weight 91 kDa
Observed molecular weight 70 kDa, 100 kDa
GenBank accession numberNM_013261
Gene Symbol PGC1a
Gene ID (NCBI) 10891
RRIDAB_2828002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBK2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.