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KD/KO Validated

PGK1 Polyclonal antibody

Name: PGK1 Polyclonal antibody
Brand: Proteintech
SKU: 17811-1-AP

PGK1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17811-1-AP

Synonyms

MIG10, OK/SW cl.110, PGK1, PGKA, phosphoglycerate kinase 1, Primer recognition protein 2, PRP 2

Validation Data Gallery

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AllWBIP

WB analysis using 17811-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17811-1-AP
WB result of PGK1 antibody (17811-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGK1 transfected HeLa cells.
WB analysis of HEK-293 using 17811-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17811-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17811-1-AP (PGK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of SW 1990 using 17811-1-AP
IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:2000) with SW 1990 cells lysate 2800 ug.
IP experiment of HeLa using 17811-1-AP
IP result of anti-PGK1 (IP:17811-1-AP, 4ug; Detection:17811-1-AP 1:10000) with HeLa cells lysate 980 ug.

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Cat No. 17811-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HepG2 cells, HEK-293 cells, HeLa cells, PC-12 cells, PC-3 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	SW 1990 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 71 publications below
IHC	See 18 publications below
IF	See 5 publications below
IP	See 5 publications below
CoIP	See 4 publications below

Product Information

17811-1-AP targets PGK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PGK1 fusion protein Ag12119 相同性解析による交差性が予測される生物種
Full Name	phosphoglycerate kinase 1
Calculated molecular weight	417 aa, 45 kDa
Observed molecular weight	40-45 kDa
GenBank accession number	BC103752
Gene symbol	PGK1
Gene ID (NCBI)	5230
RRID	AB_2161218
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

Protocols

Product Specific Protocols
WB protocol for PGK1 antibody 17811-1-AP	Download protocol
IP protocol for PGK1 antibody 17811-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Transcriptional Regulation of the Warburg Effect in Cancer by SIX1. Authors - Ling Li View Article
mouse	IF	Nat Neurosci A glycolytic shift in Schwann cells supports injured axons. Authors - Elisabetta Babetto View Article
human	WB,IF,IP	Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. Authors - Youming Zhu View Article
human	WB	Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Authors - Xiao-Hui Wang View Article
human	WB	Nat Commun Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression. Authors - Mei-Lin Weng View Article
mouse	IF	Cardiovasc Res Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism Authors - Yang Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PGK1 Polyclonal antibody

PGK1 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 17811-1-AP

WB analysis of HeLa using 17811-1-AP

WB analysis of HEK-293 using 17811-1-AP

WB analysis of HepG2 using 17811-1-AP

IP experiment of SW 1990 using 17811-1-AP

IP experiment of HeLa using 17811-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

A glycolytic shift in Schwann cells supports injured axons.

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression.

Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism